Antimicrobial activity of crude extracts of three ...

Ethanol Extract Six grams of the powdered plant material was extracted in a soxhlet extractor using 25ml of 40% ethanol. The extract was filtered using What man filter paper and the filtrates were then evaporated and dried at 60oC in vacuo. The resulting extracts were subsequently weighted to produce 0.034, 0.035 and 0.039g of A B and C

Evaluation of diffusion and dilution methods to determine ...

The aim of this study was to evaluate diffusion and dilution methods for determining the antibacterial activity of plant extracts and their mixtures. Several methods for measurement of the minimal inhibitory concentration (MIC) of a plant extract are available, but there is …

Journal of Sciences Preparation of Plant Extracts from ...

Figure 6: concentration of extracts using rotary evaporator and laboratory hot plate Table 1: Percent extract yield from three indigenous medicinal plants Plants/percentage yield Root extracts Stem extracts Vernonia amygdalina 13.6 11.96 Gongronema latifolia 10.75 10.01 Nauclea latifolia 19.90 18.30 3. RESULTS AND DISCUSSION

Effect of plant extracts and essential oils on root-knot ...

lower concentration, and its activity against that nematode was not improved by increasing the con-centration. Fruit extract of P. anisum at the high-er concentration was as effective as was extract of O. syriacum. With M. incognita, the tenfold concentration of these plant extracts caused an increase in J2 mor-tality after 24 and 72 h.

Corrosion Control of Aluminium Alloy in Alkaline Solution ...

guineense leave extracts inhibited the corrosion of aluminium in the test solutions and that the inhibition efficiency depended on the concentration of the plant extract as well as on the time of exposure of the aluminium samples in 0.25M NaOH solu-tions containing the extracts.

Antimicrobial activity of some plant extracts against ...

All plant extracts was potentially effective against S. typhi, E. coli, B. cereus and S. aureus implicated in food spoilage but the extract of Punica granatum was the most effective extract with concentration of 500 mg/ml. Ethanolic P. granatum peels extracts was found to be potentially effective against Micrococcus luteus, S. aureus, Bacillus ...

Effect of Antimicrobial Activities on the Various Solvents ...

ml) of plant extracts were pipetted and lowest concentration of extracts at which dropped to the sample discs. The sample no colony was observed after incubation discs were subsequently placed on a (no macroscopic visible growth). prepared fungal SDA plates.

Suppression of Nematode Pests of Tomato with Aqueous Leaf ...

Preparation and Application of Plant Extracts:The results presented in Table 1 show that the leaf Extracts were prepared from the leaves of nitta, tobacco extracts applied at different concentration (50% and ) and pawpaw. Each of these leaves was collected within suppressed galls on the root of tomato thereby attempted

IN VITRO SCREENING OF METHANOL PLANT EXTRACTS FOR …

ANTIBACTERIAL ACTIVITY OF METHANOL PLANT EXTRACTS 535 Minimum inhibitory concentration: The minimum inhibitory concentration (MIC) of the extracts was measured by agar dilution method (Nostro et al., 2000) and agar well diffusion method (Basu et al., 2005). a.

How do plant extracts work? - DSM

How do plant extracts work? Herbs, spices, plant extracts and essential oils (EO) have been used for centuries to preserve food for humans and to make it more appetizing. These products are well recognized for their ... and concentration of the product obtained. Because of the large

Minimum Inhibitory Concentration (MIC) of antimicrobial ...

Minimum Inhibitory Concentration (MIC) of antimicrobial activity in various dried extracts of Gnaphalium polycaulon, Indian folkloric medicinal plant K. Shanmugapriya*, T. Senthil Murugan and Tha Thayumanavan School of Biotechnology, Dr. G. R. Damodaran College of Science, Coimbatore - 641014, Tamil Nadu, India.

Plant Extracts Concentration And Refining | Organic ...

Plant extracts are extracted by solvent and enriched by means of concentration; these plant extracts are most sensitive. Typical plant extracts include traditional Chinese …

Dosage calculations & extract equivalency in herbal ...

concentration steps employed (eg. none, heat, low pressure) ... in most cases it is desirable to create an extract which most closely represents the chemical complexity of the dry plant material. Such extracts are often termed "Galenical extracts", and more recently "full-spectrum extracts".

Assessments of Antibacterial Effects of Aqueous-Ethanolic ...

Even for the other tested bacteria at the same concentration (62.5mg/ml), little effects were shown. The mean zones of inhibition of the plant extract at the concentration of 500 mg/ml for all the tested bacteria were greater than the lowest concentration of the plant extract (62.5mg/ml) and …

Plant extract: Neem | Infonet Biovision Home.

Plant extract: Neem. Email this page; Neem seeds ... Unlike most botanical insecticides, neem also has a somewhat "systemic" effect. This means that plants can take up neem extracts through their roots and leaves, spreading the material throughout the plant tissues. ... The effect of neem as a pesticide depends on the concentration of the ...

PLANT PIGMENT ANALYSIS: - Michigan State University

part 2. characterize the light-absorbing properties of your plant extract - generate an absorbance spectrum and determine amax. the wavelength at which your plant extract absorbs the maximum amount of light. epxlal. prepare a sample cuvette.

Invitro cytotoxicity study on combined plants extracts ...

Different extracts of the plant exhibited different activity on different cell lines. This selectivity could be due to the sensitivity of the cell line to the active compounds in the extract or to tissue specific response. The effect of the samples on the proliferation of HT-29 was expressed as the % cell viability.

2.0 MATERIALS AND METHODS 2.1 Plant material and extract ...

A stock solution of plant aqueous extract was prepared by dissolving 100 mg of extract in 1 ml of sterile distilled water. Likewise, for the ethanol extract, 100 mg of extract was dissolved in 1 ml of 10 % Tween-20 rather than the original solvent (i.e. Dimethyl sulfoxide), and so the initial concentration of the plant extract (100 mg/ml)

Evaluation of antimicrobial activity of different solvent ...

using sterile cork borer. Stock solution of each plant extract was prepared at a concentration of 1 mg/ml in different plant extacts viz. Methanol, Ethanol, Petroleum Ether, Water. About 100 µl of different concentrations of plant solvent extracts were added sterile syringe into the wells and allowed to diffuse at room temperature for 2hrs.

PHYTOCHEMICAL ANALYSIS AND DETERMINATION OF TOTAL ...

Preparation of Extracts of Plant Material: Plant extracts of each plant were prepared using distilled water as extracting solvent: A. Water extract of Hedychium spicatum: 44g of the dried and powdered plant material (r hizome) was extracted with 440ml of distilled water …

Enzyme Extraction - Welcome | Plant Pathology and Plant ...

Add 1~50 ul of the crude extract to the reaction mixture, vortex, and record the start point of time. After certain amount of time (eg. 20 min for GST, 5 min for peroxidase), transfer the reaction samples to the cuvettes.

Available online a t ...

extracts that have different zones of inhibition and then selecting the lowest concentration of each extract (Agaetmor, 2009). Determination of minimum inhibitory concentration (MIC) The minimum inhibitory concentration of the plants that showed inhibition in the antimicrobial screening was used.

ANTIBACTERIAL ACTIVITY OF MEDICINAL PLANT EXTRACTS

represent the inhibitory capacity of each plant extract against the bacteria tested, were obtained and expressed as %v/v and mg/mL (Table 2). Table 1. Characteristics of the plants extracts. Scientific name Common name Part of the plant used Extract dry weight (mg/mL) Allium sativum Garlic Bulbs 133.0 Caryophyllus aromaticus Clove Flower buds 95.0

TOTAL PHENOLIC CONTENT, FLAVONOID …

to examine the total phenolic content, flavonoid concentration and antioxidant activity. The yield of extract obtained from 10 g of dry plant material was measured for each extract (Table 1). The highest yield of solid residue was obtained using water or methanol as extraction solvents. Table 1.

PHYTOCHEMICAL ANALYSIS DETERMINATION OF VITAMINS A, B1, B2 ...

concentration values of the phytochemicals in mg/100g were flavonoids 4.266 ... Herbalism is a traditional medicine or folk medicine practice based on the use of plants and plant extracts. It is also known as botanical medicine, medical herbalism, herbal medicine, herbology and herblore

How can we determine the concentration of an extract after ...

Here the word concentration is not suitable but it would be considered plant extract or herbal extract. It still retains some of the solvent (3-1%) which is difficult to be removed.

Evaluation of Selected Medicinal Plants Extracted in ...

60 Evaluation of Selected Medicinal Plants Extracted in Different Ethanol Concentrations for Antibacterial Activity against Human Pathogens . Chitra Wendakoon 1*, Peter Calderon2, and Daniel Gagnon2 . 1Food Safety Laboratory, Southwest Center for Animal Health, Food safety and Bio-security, . College of Agricultural, Consumer and Environmental Sciences, New Mexico State University,

How can I determine plant extract concentration?

I understand that you want to prepare a stock solution (s.s.) concentration from the plant extract to be examined by dilutions in a final concentration with keeping the percent of DMSO as 0.5%.